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In vitro Fertilization of Bovine Oocytes Exposed to Bovine Herpesvirus 1 (BHV‐1)
Author(s) -
Bielanski A.,
Dubuc C.
Publication year - 1993
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.1993.tb00998.x
Subject(s) - blastocyst , bovine herpesvirus 1 , oocyte , andrology , in vitro , in vitro maturation , embryo , biology , human fertilization , oviduct , in vitro fertilisation , chemistry , virus , embryogenesis , virology , herpesviridae , anatomy , medicine , microbiology and biotechnology , genetics , viral disease
Contents: Cumulus‐oocyte complexes were matured for 24 hours in the presence or absence of bovine herpes virus‐1 (BHV‐1, 10 6 TCID 50 /ml), fertilized in vitro and then co‐cultured on oviductal cells to the blastocyst stage. The percentages of oocytes which cleaved were 48% (n = 905) for oocytes matured in the presence of BHV‐1, and 51% (n = 1004) for the control oocytes. Comparing BHV‐1‐treated to control, the percentages of cleaved oocytes which developed to the blastocyst stage were 29% and 31%, respectively (p > 0.05). Embryos produced from the experimental group of oocytes tested positive for BHV‐1.