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Freezing of Maiden Stallion Semen ‐ Motility and Morphological Findings in Sperm Cells Assessed by Various Staining Methods Including a Monoclonal Antibody with Reactivity Against an Antigen in the Acrosomal Ground Substance
Author(s) -
Wockener A.,
Schuberth H.J.
Publication year - 1993
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.1993.tb00995.x
Subject(s) - semen , staining , sperm , andrology , monoclonal antibody , antigen , biology , immunofluorescence , antibody , motility , ground substance , chemistry , immunology , anatomy , medicine , connective tissue , microbiology and biotechnology , genetics
Contents: From a group of thirteen 3‐year‐old Hanoveranian maiden stallions ejaculates were collected on 5 consecutive days and frozen each in 3 different packaging forms. Fresh, resuspended and frozedthawed semen was tested for motility and morphology with special regard to acrosomal integrity. Concerning packaging form no differences were found between 4 ml maxi straw, flattened 1.7 ml straw and 0.5 ml straw. The comparison of 3 direrent conventional staining techniques with an immunofluorescence test, employing a monoclonal antibody against an antigen in the acrosomal ground substance, revealed that staining sperm cells with the antibody yielded far more reproducible and consistent results ‐ especially in diluted semen ‐ than staining with Karras, moded Karras or Spermac®.