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Morphology of Day‐7 Bovine Demi‐Embryos and their Post‐transfer Viability *
Author(s) -
Albihn A.,
Gustafsson H.,
RodriguezMartinez H.
Publication year - 1991
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.1991.tb01521.x
Subject(s) - embryo , andrology , incubation , biology , embryo transfer , morphology (biology) , anatomy , in vitro , microbiology and biotechnology , zoology , genetics , biochemistry , medicine
Contents: Day 7 bovine embryos were microsurgically bisected and replaced into surrogate zonae pellucidae. They were fixed immediately after bisection and at various intervals of in vitro incubation at 35 °C in modified Dulbecco's medium. At the light microscopical level, the bisected embryos restored the prebisection morphology within 30 min. after splitting. The electron microscopy confirmed these findings, suggesting that day 7 bovine demi‐embryos for transfer purposes, should be cultured for 30 min before morphologically evaluated. Eleven pairs of bisected day 7 bovine embryos were transferred to 11 synchronized heifers. The recipient heifers were slaughtered at day 15, and the recovered embryos evaluated. Nine of the demi‐embryos developed to morphologically, normal spherical to elongated, embryos .