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Experiences of Using PCR for Sexing Bovine Embryos *
Author(s) -
Bredbacka P.,
Bredbacka K.,
Peippo J.
Publication year - 1991
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.1991.tb01518.x
Subject(s) - sexing , embryo , polymerase chain reaction , biology , andrology , dna , microbiology and biotechnology , genetics , gene , medicine
Contents: Bovine embryos were used to evaluate the accuracy and the efficiency of sexing embryos by amplifying male specific DNA using the polymerase chain reaction. Blind tests with biopsies and biopsied embryos suggested that the method is accurate. However, often the amplification was unsuccessful and the assay therefore uninformative. We were able to amplify male specific DNA from degenerated embryos as well as from embryos stained with Hoechst 33342 .