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Redefinition of the map position and validation of a major quantitative trait locus for fire blight resistance of the pear cultivar ‘Harrow Sweet’ ( Pyrus communis L.)
Author(s) -
Le Roux PierreMarie F.,
Christen Danilo,
Duffy Brion,
Tartarini Stefano,
Dondini Luca,
Yamamoto Toshiya,
Nishitani Chikako,
Terakami Shingo,
Lespinasse Yves,
Kellerhals Markus,
Patocchi Andrea
Publication year - 2012
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2012.02000.x
Subject(s) - quantitative trait locus , fire blight , biology , pear , harrow , cultivar , pyrus communis , malus , locus (genetics) , horticulture , botany , genetics , agronomy , erwinia , gene
With 3 figures and 2 tablesAbstract In a previous study, a QTL analysis was conducted on a pear F 1 progeny derived from a cross ‘Passe Crassane’ (PC) × ‘Harrow Sweet’ (HS). Four genomic regions associated with fire blight resistance were identified, including two main QTL located on linkage groups (LGs), 2A and 4 of ‘Harrow Sweet’ (HS02A and HS04). In the present study, we report the combination of LGs HS02A and HS02B into a single LG by mapping additional SSR loci from Malus or Pyrus spp. We could thereby precisely identify a single major QTL on LG HS02. We also confirm a putative QTL on LG HS04 by including new SSR markers to the pre‐existing LG HS04. Based on SSR marker analysis of ‘Harrow Sweet’ pedigree, the major HS02 QTL is presumed to originate from the cultivar ‘Early Sweet’, while the HS04 QTL was traced from ‘Harrow Sweet’ back to ‘Bartlett’. We also describe the validation of the major HS02 QTL for the fire blight severity trait in a second F 1 progeny derived from a cross ‘Angelys’ × ‘Harrow Sweet’.