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Development of a high resolution melting (HRM) marker linked to genic male sterility in Capsicum annuum L.
Author(s) -
Lee HeungRyul,
An Hye Jung,
Yang Dong Cheol,
Choi Soon Ho,
Kim HyounJoung,
Rhee Han Gheol,
Harn Chee Hark
Publication year - 2012
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2012.01956.x
Subject(s) - sterility , bulked segregant analysis , biology , pepper , amplified fragment length polymorphism , capsicum annuum , high resolution melt , genetics , molecular marker , cytoplasmic male sterility , genetic marker , primer (cosmetics) , ecori , horticulture , gene mapping , chromosome , restriction enzyme , genotype , gene , population , chemistry , demography , organic chemistry , sociology , genetic diversity
With 4 figures and 1 tableAbstract The characteristics of male sterility (MS) are used in breeding programmes to achieve economical seed production. Male sterility is divided into genic male sterility (GMS) and cytoplasmic male sterility (CMS), which are used to breed commercial pepper varieties. The CMS system, however, is not feasible in some pepper varieties, including bell peppers, because of the absence of a restorer source. GMS is thus important for seed production in bell peppers. In this study, a GMS‐linked marker from bell peppers was developed using the bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) method using F 2 and sibling individuals. We used 1024 AFLP primer sets and found a polymorphism from Eco RI ACG/ Mse I GTT among the siblings. An internal sequence‐based primer was designed from the 395 bp sequence for high‐resolution melting (HRM) analysis, and the marker score of 87 of 92 F 2 individuals corresponded to their phenotypes. The marker was mapped on chromosome 5 on the AC99 map.