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Improved production of doubled haploids in Brassica rapa through microspore culture
Author(s) -
Zhang Yun,
Wang Aijie,
Liu Yang,
Wang Yushu,
Feng Hui
Publication year - 2012
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2011.01927.x
Subject(s) - microspore , brassica rapa , biology , doubled haploidy , ploidy , microspora , botany , embryogenesis , embryo , somatic embryogenesis , brassica , microbiology and biotechnology , stamen , gene , genetics , pollen
With 1 figure and 7 tablesAbstract The current study aims at producing doubled haploid Brassica rapa plants through isolated microspore culture. Genotypic differences in embryogenic response and regenerative ability of microspore‐derived embryos to plants were observed. Compared with the longer incubation period (72 h), heat shock treatment at 33°C for 24 or 48 h was more beneficial for microspore embryogenesis. Microspore embryogenesis was improved with the addition of 6‐benzylaminopurine and naphthalene acetic acid to the NLN medium. The highest frequency of microspore embryogenesis was achieved in ‘SH‐8’ (21.1 embryos/bud), whereas ‘SH‐6’ had the best regeneration ability (67.4%). In total, 1112 regenerated plants were produced from 2025 embryos in a series of experiments using different modifications of microspore culture technique. The average spontaneous doubling frequency of the different genotypes of B. rapa was 57.5%.