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Improving cotton ( Gossypium hirsutum L.) plant resistance to reniform nematodes by pyramiding Ren 1 and Ren 2
Author(s) -
Fang David D.,
Stetina Salliana R.
Publication year - 2011
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2011.01889.x
Subject(s) - biology , rotylenchulus reniformis , gossypium , pest analysis , gossypium hirsutum , resistance (ecology) , nematode , population , microsatellite , gene , botany , agronomy , genetics , allele , meloidogyne incognita , ecology , demography , sociology
With 4 tablesAbstract Reniform nematode ( Rotylenchulus reniformis ) has become a major pest of cotton in the mid‐south area of the United States. Resistance genes, Ren 1 and Ren 2 from Gossypium longicalyx and Gossypium aridum , respectively, have been identified and introduced into Upland cotton ( Gossypium hirsutum ). We developed an F 2 population of 184 progeny plants by crossing Ren 2 ‐containing plants with a LONREN‐2 plant that had the resistance gene Ren 1 . The F 2 plants were evaluated for their resistance to reniform nematodes in a growth chamber. Microsatellite markers BNL2662 and BNL3279 were analysed to assist the selection of proper parental plants and F 1 progeny and to study the segregation of the resistance genes in the F 2 population. Our results suggested that Ren 1 and Ren 2 were duplicate genes with Ren 1 residing on chromosome 11 (A subgenome) and Ren 2 on chromosome 21 (D subgenome). F 2 plants containing either Ren 1 or Ren 2 had significantly fewer nematodes than the susceptible Upland cotton genotype. No significant difference in nematode resistance was found between plants containing Ren 1 and those having Ren 2 , indicating that these two genes may have similar resistance mechanisms. Plants containing both Ren 1 and Ren 2 appeared to have higher resistance than those with just one of the genes, and pyramiding these two genes may be a valuable tool to cotton breeders when managing this pest.