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Identification and characterization of microsatellite markers from Musa balbisiana
Author(s) -
Wang Jingyi,
Huang Bingzhi,
Chen Yeyuan,
Feng Suping,
Wu Yaoting
Publication year - 2011
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2011.01861.x
Subject(s) - biology , microsatellite , ploidy , subspecies , loss of heterozygosity , locus (genetics) , genetics , allele , botany , gene , zoology
With 2 figures and 2 tablesAbstract Microsatellite sequences were determined from Musa balbisiana , a wild diploid species in China, using selectively amplified microsatellite analysis. Fourteen microsatellite loci were evaluated in a collection of 22 cultivated bananas and 11 wild species. A total of 77 alleles were detected with an average of 5.5 alleles and heterozygosity (H) = 0.355 per locus. Efficient loci transferability of M. balbisiana simple sequence repeat markers across other wild species of the genus was observed. Sequencing alleles at four loci showed that, apart from microsatellite length variation, point mutations and insertions/deletions were quite abundant, especially when comparing Musella lasiocarpa and Ensete glaucum with the other Musa species/subspecies. In the sequenced regions, some single‐nucleotide polymorphisms were identified which distinguished M. lasiocarpa and E. glaucum from the other Musa species.