Premium
Production of novel alloplasmic male sterile lines in Allium cepa harbouring the cytoplasm from Allium roylei
Author(s) -
Vu Hao Q.,
Iwata Machiko,
Yamauchi Naoki,
Shigyo Masayoshi
Publication year - 2011
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2011.01855.x
Subject(s) - backcrossing , allium , pollen , biology , cytoplasmic male sterility , botany , bulb , sterility , horticulture , genetics , gene
With 3 figures and 5 tablesAbstract To develop alloplasmic lines of Allium cepa , cytoplasmic substitution by continuous backcrossing was performed using Allium roylei as a cytoplasm donor. The chromosomes of a single F 1 plant between A. roylei (♀) and shallot were doubled, and then backcrossing with shallot was performed to produce BC 1 as allotriploids. One allotriploid plant was used for backcrossing with bulb onion to produce BC 2 with 2n = 16, 17, 23 and 24. The BC 2 plants possessing 16 chromosomes were checked for pollen fertility in Yamaguchi, Japan (N34°11′), and then backcrossed with bulb onion again to evaluate their seed‐setting characteristics. The pollen fertility of BC 2 ranged from 0% to more than 10%. A large number of plants showed no pollen fertility (0%). In Kagawa and Hokkaido, most of the plants were completely pollen sterile, while the percentages of seed sets in BC 2 were high enough to reproduce BC 3 seeds. The results of this study revealed that an exploitation of A. roylei cytoplasm would be useful for the development of a novel cytoplasmic male sterility line in Allium .