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Identification and gene mapping of a soybean chlorophyll‐deficient mutant
Author(s) -
Zhang H.,
Zhang D.,
Han S.,
Zhang X.,
Yu D.
Publication year - 2011
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2010.01844.x
Subject(s) - biology , mutant , chlorophyll , chloroplast , nuclear gene , gene , ethyl methanesulfonate , photosynthesis , botany , seedling , variegation (histology) , genetics , genome
With 5 figures and 2 tablesAbstract Chlorophyll variation mutants are important materials for studying chlorophyll metabolism, photosynthesis, chloroplast biogenesis and related physiological processes. In this study, a chlorophyll‐deficient soybean mutant ( cd1 ) was isolated by screening M 2 progenies of cultivar ‘ Nannong 86‐4 ’ treated with 0.4% ethyl methanesulfonate. cd1 displayed yellow‐green leaves from seedling stage V1 to fully flowering stage R2, abnormal chloroplasts, lower total chlorophyll content (30–66% reduction), lower plant height and lower seed yield compared to its wild‐type control. Genetic analysis demonstrated that the visible yellow‐green trait was controlled by a single recessive nuclear gene. Subsequently, the cd1 ‐mutant gene was mapped at a 6.9‐cM interval on chromosome 10, flanked by two simple sequence repeat markers Satt633 and Sat_291. These results provide us with the opportunity to study a novel gene regulating chlorophyll synthesis.