Premium
A linkage map of pak‐choi ( Brassica rapa ssp. chinensis ) based on AFLP and SSR markers and identification of AFLP markers for resistance to TuMV
Author(s) -
Xinhua W.,
Yang L.,
Huoying C.
Publication year - 2011
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2010.01811.x
Subject(s) - amplified fragment length polymorphism , biology , genetics , genetic linkage , brassica rapa , microsatellite , genetic marker , xanthomonas campestris , gene , population , genetic diversity , allele , demography , sociology
With 2 figuresAbstract A genetic linkage map for pak‐choi, a very important economical and nutritional vegetable crop in Asia, has been constructed using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers based on 180 F 2 individuals. It includes 133 AFLP and 74 SSR markers from 57 AFLP and 65 SSR primer combinations, respectively. Fifty SSR markers were previously used to construct a framework map and all AFLP markers and another 24 SSR markers were inserted. The linkage map consists of 10 linkage groups with a total distance of 1123 cM and an average interval of 5.43 cM. Turnip mosaic virus (TuMV) is one of the most important pathogens of pak‐choi. TuRBCH01 , a TuMV‐C 5 resistance gene, was successfully mapped into R6 linkage group in the present genetic linkage map and was positioned between AFLP markers E36M62‐3 and E44M48‐1.