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Identification of a null allele at the Wx‐A1 locus in durum wheat ( Triticum turgidum L. ssp. durum Desf.)
Author(s) -
Vanzetti L.S.,
Pflüger L.,
Bainotti C.T.,
Jensen C.,
Helguera M.
Publication year - 2010
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2009.01741.x
Subject(s) - biology , genetics , allele , null allele , locus (genetics) , gene , exon , triticum turgidum
With 3 figures and 1 tableAbstract This work describes a novel null Wx‐A1 allele detected in one cultivar and two breeding lines from a collection of 48 durum wheats from Argentina. The electrophoretic patterns of waxy proteins showed 45 accessions displaying Wx‐A1 and Wx‐B1 proteins and three accessions that lacked the Wx‐A1 protein. Partial sequence of the Wx‐A1 gene obtained from the three lines lacking the Wx‐A1 protein showed a 1‐bp deletion in exon 6 that produces a frameshift and a premature stop codon in the gene sequence. In addition, a PCR‐RFLP marker suitable to detect the null Wx‐A1 allele in segregating populations is described.