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Low‐molecular‐weight glutenins in durum wheat: analysis of Glu‐A3 alleles using PCR markers
Author(s) -
Igrejas G.,
Juhász A.,
Gianibelli M. C.,
Gale K. R.,
Rahman S.
Publication year - 2010
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2009.01724.x
Subject(s) - biology , glutenin , allele , locus (genetics) , genetics , cultivar , amplicon , primer (cosmetics) , marker assisted selection , gene , molecular marker , genetic marker , polymerase chain reaction , botany , protein subunit , chemistry , organic chemistry
With 1 figure and 1 tableAbstract The aim of the present study was to compare the durum and bread wheat alleles encoded at the Glu‐A3 locus (represented by Glu‐A d 3 and Glu‐A a 3 , respectively) using PCR markers designed for bread wheat Glu‐A a 3 alleles to distinguish different durum alleles. Diversity in low‐molecular‐weight glutenin subunits (LMW‐GS) was analysed in eight cultivars representing all known Glu‐A d 3 alleles present in durum wheat. Six of the eight Glu‐A d 3 alleles of durum wheat could be distinguished using a combination of seven different primer sets based on LMW‐GS sequences from bread wheat. However, allele a (cv. ‘Mexicali’), allele b (cv. ‘Langdon’) and allele c (cv. ‘Cocorit’) of durum wheat could not be distinguished using any of the markers tested. Amplicons of identical size were produced for alleles f (cv. ‘Clarofino’) and allele g (cv. ‘Claro de Balazote’), using all except one primer set ( Glu‐A3a ), indicating genes of these alleles share a very high degree of identity. The markers described in this study are useful for the DNA‐based identification of durum wheat cultivars and the identification of the Glu‐A d 3 a , b , c , d , e , f , g and h alleles of durum wheat for the purposes of marker‐assisted selection.