Premium
Characterization of Pi‐ta blast resistance gene in an international rice core collection
Author(s) -
Wang X.,
Fjellstrom R.,
Jia Y.,
Yan W. G.,
Jia M. H.,
Scheffler B. E.,
Wu D.,
Shu Q.,
McClung A.
Publication year - 2010
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2009.01706.x
Subject(s) - biology , indel , gene , genetics , allele , plant disease resistance , cultivar , r gene , intron , botany , single nucleotide polymorphism , genotype
With 3 figures and 3 tablesAbstract The Pi‐ta gene in rice prevents the infections by races of Magnaporthe oryzae containing AVR‐Pita . In the present study, 1790 accessions were characterized for Pi‐ta , and Pi‐ta independent resistance genes using marker analysis, disease evaluation with the race IB‐49 carrying AVR‐Pita , and IE‐1k not carrying AVR‐Pita and sequence analysis. A total of 183 accessions were identified using a Pi‐ta ‐indel marker from the intron region. Sequence analysis revealed that resistance functional nucleotide polymorphism (FNP) was present in 163 accessions including reference cultivars. Among them, 89 were resistant to IB‐49 and susceptible to IE‐1k indicating that these accessions contain Pi‐ta R alleles. Four accessions were susceptible to IB‐49 suggesting that components were not intact in Pi‐ta ‐mediated resistance. In contrast, 14 accessions with the susceptible FNP were resistant to IB‐49 suggesting these 14 accessions may contain Pi‐ta independent new R genes. Together, 83 accessions were identified to contain new R gene to IE‐1k. These accessions were genetically distinct determined by simple sequence repeat markers. The results could impact breeding for blast resistance worldwide.