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Genetic and Molecular Characterization of Vernalization Genes Vrn‐A1 , Vrn‐B1 , and Vrn‐D1 in Spring Wheat Germplasm from the Pacific Northwest Region of the U.S.A.
Author(s) -
Santra D. K.,
Santra M.,
Allan R. E.,
Campbell K. G.,
Kidwell K. K.
Publication year - 2009
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2009.01681.x
Subject(s) - germplasm , biology , allele , genetics , vernalization , genetic diversity , single nucleotide polymorphism , genotype , cultivar , gene , botany , population , demography , sociology
The objective of this study was to determine the Vrn ‐ 1 allelic composition of spring wheat germplasm from the Pacific Northwest region of the USA. Individual plants from 56 spring wheat lines were crossed to near‐isogenic tester lines carrying the dominant allele Vrn‐A1 , Vrn‐B1 or Vrn‐D1 . F 2 progeny were evaluated for growth habit in the field and Vrn‐1 allelic composition was determined through chi‐square analysis. Lines also were analysed with DNA sequence‐based Vrn‐1 allele‐specific markers. A majority of the germplasm carried the dominant allele Vrn‐A1a alone or in combination with Vrn‐B1 , Vrn‐D1 or Vrn‐B3 alleles. Vrn‐B1 and Vrn‐D1 were almost always associated with other dominant Vrn‐1 allele(s). Based on DNA sequence analysis, a novel Vrn‐B1 allele referred to as Vrn‐B1b , which carried a single nucleotide polymorphism (SNP) and a 36 bp deletion, was identified in cultivar ‘Alpowa’. These results will be useful to wheat breeders for choosing parents with different Vrn‐1 alleles for crossing to maximize diversity at the Vrn‐1 loci with an expectation of identifying superior Vrn‐1 allelic combinations for cultivar improvement.