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Identification and molecular tagging of a stripe rust resistance gene in wheat line P81
Author(s) -
Pu Z. J.,
Chen G. Y.,
Wei Y. M.,
Yang W. Y.,
Yan Z. H.,
Zheng Y. L.
Publication year - 2010
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2009.01649.x
Subject(s) - biology , genetics , microsatellite , genotyping , gene , rust (programming language) , genetic distance , genetic marker , common wheat , genetic linkage , chromosome , genetic variation , genotype , allele , computer science , programming language
With 3 figures and 3 tablesAbstract Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat ( Triticum aestivum L.). With the objective of identifying and tagging a new gene for resistance to stripe rust in wheat line P81, F 1 , F 2 and F 2:3 populations from the cross ‘Chuanmai 28’/P81 were inoculated with Chinese PST race CYR32 in greenhouse and field trials. P81 carried a single dominant gene for resistance (designated YrP81 ) to CYR32. Tests of allelism showed that YrP81 was different from Yr5 , Yr10 , Yr15 and Yr26 . Simple sequence repeat (SSR) and resistance gene‐analogue polymorphism (RGAP) between the parents were used for genotyping the F 2 populations. YrP81 was closely linked to four SSR loci on chromosome 2BS with genetic distances of 18.3 cM ( Xwmc25 ), 1.8 cM ( Xgwm429 ), 4.1 cM ( Xwmc770 ) and 5.3 cM ( Xgwm148 ). Two RGAP markers RGA1 (NLRR/XLRR) and RGA2 (Pto kin4/NLRR‐INV2) were also closely linked to YrP81 with genetic distances of 4.7 and 6.3 cM, respectively. The linkage map of YrP81 and molecular markers was established in the order Xwmc25 ‐ RGA2 ‐ RGA1 ‐ Xgwm429 ‐ YrP81 ‐ Xwmc770 ‐ Xgwm148 . Pedigree analysis, response patterns with Chinese PST races and associations with markers suggested that YrP81 is a novel stripe rust resistance gene. The PCR‐based microsatellite and RGAP markers identified here could be applied in selection of YrP81 in wheat breeding.