Premium
A codominant SCAR marker linked to the genic male sterility gene ( ms 1 ) in chili pepper ( Capsicum annuum )
Author(s) -
Lee J.,
Yoon J. B.,
Han J.H.,
Lee W. P.,
Do J. W.,
Ryu H.,
Kim S. H.,
Park H. G.
Publication year - 2010
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2009.01643.x
Subject(s) - biology , bulked segregant analysis , amplified fragment length polymorphism , sterility , pepper , locus (genetics) , genetic marker , genetics , molecular marker , hybrid seed , cleaved amplified polymorphic sequence , population , cytoplasmic male sterility , capsicum annuum , marker assisted selection , primer (cosmetics) , rapd , gene , gene mapping , horticulture , restriction fragment length polymorphism , genotype , chromosome , hybrid , genetic diversity , demography , organic chemistry , sociology , chemistry
With 3 figuresAbstract As one of the genic male sterility (GMS) materials in chili pepper ( Capsicum annuum L.), GMS1 has been used for commercial F 1 hybrid seed production. The male sterility of GMS1 is controlled by a recessive nuclear gene, named ms 1 . In this study, we developed DNA markers linked to the ms 1 locus using a combination of bulked segregant analysis and amplified fragment length polymorphism (AFLP) in a segregating sibling population. From the screening of 1024 AFLP primer combinations, the AFLP marker E‐AGC/M‐GTG (514 bp) was identified as being linked to the ms 1 locus at a distance of about 3 cM. Based on internal sequencing analysis of the E‐AGC/M‐GTG marker between male fertile and sterile plants, we identified three small deletions with a size of altogether 42 bp in the male‐fertile plant and developed a codominant sequence characterized amplified region (SCAR) marker. This SCAR marker may be valuable for marker‐assisted breeding in the hybrid seed production system of chili pepper using the GMS1 line.