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Rye introgression lines as source of alleles for pollen‐fertility restoration in Pampa CMS
Author(s) -
Falke K. C.,
Wilde P.,
Miedaner T.
Publication year - 2009
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01589.x
Subject(s) - introgression , biology , backcrossing , pollen , cytoplasmic male sterility , fertility , sterility , genetics , hybrid , chromosome , hybrid seed , allele , microsatellite , botany , gene , population , demography , sociology
Restoration of pollen‐fertility is required to guarantee successful seed production of hybrid varieties based on cytoplasmatic male‐sterility (CMS) induced by Pampa cytoplasm. As most European restorer lines have still numerous disadvantages, using genetic resources via introgression libraries might be promising to identify and localize new and more effective restorer genes. The objectives of this study were to (1) evaluate in field trials the performance of 39 candidate introgression lines (pre‐ILs) for pollen‐fertility restoration and (2) to identify donor chromosome segments (DCS) underlying pollen‐fertility restoration. Exotic alleles from the Iranian primitive rye accession Altevogt 14160 were introduced into the elite non‐restorer inbred line L2053‐N by marker‐assisted backcrossing. BC 2 S 3 pre‐ILs were genotyped using amplified fragment length polymorphisms and simple sequence repeats. Pollen‐fertility restoration was evaluated in replicated field trials across two testers at two locations in 2006 for analysing the phenotypic effects of the exotic DCS. Out of 39 pre‐ILs, we determined three showing full pollen‐fertility across both testers and locations carrying a DCS on chromosome 4R. Moreover, one pre‐IL with partial pollen‐fertility was detected showing a DCS on chromosome 1R. We conclude that our introgression library can serve as a valuable resource for mining favourable genes from exotic primitive rye, which can greatly promote hybrid rye breeding.