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Development of PCR‐based markers for a major locus conferring powdery mildew resistance in mungbean ( Vigna radiata )
Author(s) -
Zhang M. C.,
Wang D. M.,
Zheng Z.,
Humphry M.,
Liu C. J.
Publication year - 2008
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01521.x
Subject(s) - biology , powdery mildew , microsatellite , locus (genetics) , restriction fragment length polymorphism , genetics , vigna , genetic marker , sequence tagged site , plant disease resistance , bacterial artificial chromosome , gene mapping , molecular marker , expressed sequence tag , marker assisted selection , gene , allele , chromosome , polymerase chain reaction , botany , genome
Powdery mildew (PM) can cause significant yield loss in mungbean and several loci conferring resistance to this disease have been identified. A restriction fragment length polymorphism (RFLP) marker (VrCS65) linked closely to one of these loci was used to screen a mungbean bacterial artificial chromosome (BAC) library and positive BAC clones identified were used to develop simple sequence repeat (SSR or microsatellite) and sequence tagged site (STS) markers. Four of the new PCR markers (including two SSRs and two STSs) co‐segregated with the original RFLP marker VrCS65, and another SSR marker (VrCS SSR2) was located 0.5 cM away from it. These PCR‐based and locus‐specific markers could be useful in breeding cultivars with enhanced resistance to PM and in the further characterization of the locus including the isolation of gene(s) responsible for the resistance.