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Identification of the spontaneous 7BS/7RL intergenomic translocation in one F 1 multigeneric hybrid from the Triticeae tribe
Author(s) -
Carvalho A.,
Martín A.,
HeslopHarrison J. S.,
GuedesPinto H.,
LimaBrito J.
Publication year - 2009
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01519.x
Subject(s) - biology , triticeae , chromosomal translocation , triticale , hybrid , chromosome , genetics , genome , hordeum vulgare , powdery mildew , fluorescence in situ hybridization , aegilops , elymus , botany , poaceae , gene
The F1 AABBRH ch hybrids studied here were produced by crosses between the Portuguese triticale cultivar ‘Douro’ (AABBRR) and the tritordeum line HT9 (AABBH ch H ch ). Fluorescent in situ hybridization performed with genomic DNA probes genomic in situ hybridization (GISH) from rye and Hordeum chilense allowed the unequivocal parental genomes discrimination in all hybrids. Among 55 plants, one presented a spontaneous wheat–rye translocation which was successfully detected after GISH. Recombinant chromosomes identification was made after reprobe with pTa71 and pSc119.2. Nine rDNA loci were detected by pTa71 and pSc119.2 identified the chromosome arms involved in the translocation, after comparing the observed hybridization patterns with those described by several authors. We identified the spontaneous wheat–rye translocation as being the 7BS/7RL. Many wheat–rye translocations have been found (e.g. 1BL.1RS and 1AL.1RS), but as far as we know, this is the first time that this translocation is reported. We considered it helpful for wheat breeding programmes as it could provide the transference of interesting agronomic characteristics from rye (e.g. leaf rust resistance) to wheat.