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Production and characterization of an amphidiploid line between Brassica rapa and a wild relative Diplotaxis tenuifolia
Author(s) -
Jeong B. H.,
Saga T.,
Okayasu K.,
Hattori G.,
Kaneko Y.,
Bang S. W.
Publication year - 2009
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01517.x
Subject(s) - biology , brassica rapa , hybrid , pollen , botany , embryo rescue , brassica , interspecific hybridization
Intergeneric hybridization was performed between Brassica rapa and Diplotaxis tenuifolia following embryo rescue. Twenty‐three F 1 hybrid plants were developed from the cross B. rapa × D. tenuifolia and confirmed to be amphihaploids with 21 chromosomes in mitosis. Chromosome doubling of F 1 hybrids by colchicine treatment resulted into five amphidiploid plants which exhibited (20–21) II + (0–2) I at metaphase I (MI) of pollen mother cells. Sib‐crossing and/or open‐pollination among amphidiploid plants for more than four generations resulted in the development of an ADt‐06 line with reproductive systems capable of maintaining an amphidiploid line. The ADt‐06 line was intermediate in some morphological traits between two parental species, and was characterized by a slightly pungent taste as a physiological trait. Analyses for genomic DNA confirmed that this line was a hybrid between two species. This new amphidiploid ADt‐06 line could be a useful genetic resource for the breeding of new leafy salad vegetables.