Premium
A genetic linkage map of Physocarpus , a member of the Spiraeoideae (Rosaceae), based on RAPD, AFLP, RGA, SSR and gene specific markers
Author(s) -
Sutherland B. G.,
Tobutt K. R.,
Marchese A.,
Paternoster G.,
Simpson D. W.,
Sargent D. J.
Publication year - 2008
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01505.x
Subject(s) - biology , amplified fragment length polymorphism , genetic linkage , rapd , genetics , gene mapping , genetic marker , rosaceae , linkage (software) , molecular marker , gene , botany , genetic diversity , population , chromosome , demography , sociology
Physocarpus opulifolius is a deciduous shrub native to North America belonging to the Spiraeoideae subfamily of the Rosaceae. The cultivars ‘Luteus’ and ‘Diabolo’ are grown in gardens for their ornamental foliage, golden and purple respectively. We developed a linkage map of P. opulifolius with a view to detecting markers for the leaf colour genes, which are under major gene control. A total of 162 molecular markers (128 RAPDs, 27 AFLPs, three RGA, three STS markers and one SSR) and the leaf colour genes Pur and Aur were scored in the Physocarpus progeny and used to create a linkage map covering 586.1 cM over nine linkage groups. There was an average of 18.2 markers per linkage group and a mean linkage group length of 65.1 cM. Both leaf colour genes were mapped. This is the first reported linkage map of a member of the Spireaeoideae and the mapping of a small number of transferable markers has demonstrated its utility to comparative mapping, which will complement existing comparative mapping efforts in other rosaceous subfamilies.