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Microspore mutagenesis of Brassica species for fatty acid modifications: a preliminary evaluation
Author(s) -
Ferrie A. M. R.,
Taylor D. C.,
MacKenzie S. L.,
Rakow G.,
Raney J. P.,
Keller W. A.
Publication year - 2008
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01502.x
Subject(s) - brassica , microspore , brassica rapa , doubled haploidy , biology , ploidy , oleic acid , fatty acid , erucic acid , linolenic acid , botany , linoleic acid , biochemistry , stamen , gene , pollen
A microspore mutagenesis protocol was developed for Brassica rapa , Brassica napus and Brassica juncea for the production of double haploid lines with novel fatty acid profiles in the seed oil. Freshly isolated Brassica microspores were first cultured with ethyl methane sulphonate (EMS) for 1.5 h. The EMS was removed and the microspores were then cultured according to the standard Brassica microspore culture protocol. This protocol was used to generate over 80 000 Brassica haploid/double haploid plants. Field evaluation of B. napus and B. juncea double haploids was conducted between 2000 and 2003. Fatty acid analysis of the B. napus double haploid lines showed that saturated fatty acid proportions ranged from 5.0% to 7.7%. For B. juncea , saturate proportions ranged from 5.4% to 9.5%. Of the 7000 B. rapa lines that were analysed, 197 lines had elevated oleic acid (>55%), 69 lines had reduced α‐linolenic acid (<8%) and 157 lines had low saturated fatty acid proportions (<5%), when compared with the parental lines.