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Development of multiplex PCR markers to distinguish evergreen and deciduous azaleas
Author(s) -
Mizuta D.,
Nakatsuka A.,
Kobayashi N.
Publication year - 2008
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2008.01487.x
Subject(s) - azalea , evergreen , deciduous , biology , botany , hybrid
We successfully developed new PCR‐based markers for analysing the DNA inheritance patterns of vigorous inter‐subgeneric hybrids of azalea. The hybrids were between evergreen azaleas ( Rhododendron nakaharae and its hybrids) and fragrant deciduous azaleas ( Rhododendron arborescens and Rhododendron viscosum ). We determined the sequence of the nuclear internal transcribed spacer (ITS) region in evergreen and deciduous azaleas, and found a deletion of five base pairs (bp) in evergreen azaleas compared with deciduous azaleas. Sequences for the chloroplast mat K ‐trn K region were obtained from GenBank. Primers for the multiplex PCRs were designed using these sequence data. In the multiplex PCR of the ITS region, evergreen azaleas showed a specific band of 140 bp and deciduous azaleas showed a specific band of 800 bp. In the multiplex PCR of the mat K ‐trn K region, evergreen azaleas showed a specific band of 560 bp and deciduous azaleas showed a specific band of 300 bp. These results indicate that multiplex PCR methods are useful to distinguish evergreen and deciduous azaleas easily and rapidly.