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Development of molecular markers linked to the wheat powdery mildew resistance gene Pm4b and marker validation for molecular breeding
Author(s) -
Yi Y. J.,
Liu H. Y.,
Huang X. Q.,
An L. Z.,
Wang F.,
Wang X. L.
Publication year - 2008
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2007.01443.x
Subject(s) - powdery mildew , blumeria graminis , biology , genetics , marker assisted selection , genetic marker , molecular marker , primer (cosmetics) , bulked segregant analysis , sequence tagged site , genetic distance , common wheat , plant disease resistance , gene , chromosome , gene mapping , botany , genetic variation , chemistry , organic chemistry
Powdery mildew, caused by Blumeria graminis (DC.) E.O. Speer f. sp. tritici , is an important disease in wheat ( Triticum aestivum L.). Bulk segregant analysis (BSA) was employed to identify SRAP (sequence‐related amplified polymorphism), sequence tagged site (STS) and simple sequence repeat (SSR) markers linked to the Pm4b gene, which confers good resistance to powdery mildew in wheat. Out of 240 SRAP primer combinations tested, primer combinations Me8/Em7 and Me12/Em7 yielded 220‐bp and 205‐bp band, respectively, each of them associated with Pm4b . STS‐ 241 also linked to Pm4b with a genetic distance of 4.9 cM. Among the eight SSR markers located on wheat chromosome 2AL, Xgwm382 was found to be polymorphic and linked to Pm4b with a genetic distance of 11.8 cM. Further analysis was carried out using the four markers to investigate marker validation for marker‐assisted selection (MAS). The results showed that a combination of the linked markers STS −241 , Me8/Em7 −220 and Xgwm382 could be used for marker‐assisted selection of the resistance gene Pm4b in wheat breeding programmes.