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Development of a cDNA‐AFLP derived CAPS marker co‐segregating with the powdery mildew resistance gene Mlg in barley
Author(s) -
Korell M.,
Eschholz T. W.,
Eckey C.,
Biedenkopf D.,
Kogel Marc K.H.,
Friedt W.,
Ordon F.
Publication year - 2008
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2007.01381.x
Subject(s) - powdery mildew , biology , genetics , amplified fragment length polymorphism , molecular marker , blumeria graminis , gene , complementary dna , marker assisted selection , genetic marker , population , plant disease resistance , botany , demography , sociology , genetic diversity
Powdery mildew is an important pathogen of barley in many barley‐growing areas. To identify molecular markers for the resistance gene Mlg located on chromosome 4H, a cDNA‐AFLP approach was carried out on near‐isogenic lines. Based on the identification of a differentiating fragment of 37 bp, which turned out to be part of a nucleoside diphosphate kinase as estimated by blastn , a CAPS marker has been developed, which co‐segregated with Mlg in a population of 738 F 2 ‐plants. Due to its co‐dominant inheritance, clear banding pattern and close linkage this marker is well suited for marker‐based selection procedures.