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Cytogenetic analysis of F 1 , F 2 and BC 1 plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization
Author(s) -
Wei W. H.,
Zhang S. F.,
Wang L. J.,
LI J.,
Chen B.,
Wang Z.,
Luo L. X.,
Fang X. P.
Publication year - 2007
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2007.01370.x
Subject(s) - biology , brassica oleracea , hybrid , sinapis , pollen , genome , chromosome , botany , ploidy , brassica , genetics , gene
By intergeneric sexual hybridization between Sinapis alba and Brassica oleracea , F 1 , F 2 and BC 1 progeny plants were produced. S. alba plants (genome SS, 2n = 24) were pollinated with B. oleracea (genome CC, 2n = 18), and the fertile F 1 plants were pollinated with B. oleracea to obtain BC 1 plants. GISH analysis showed that 10 out of 12 F 1 plants had 12 S. alba chromosomes (one full S chromosome set) and nine B. oleracea chromosomes (one C chromosome sets), representing the expected hybrids. However, two F 1 plants had 12 S chromosomes and 18 C chromosomes (two C chromosome sets), indicating unexpected hybrids. A maximum of three trivalents between C and S chromosomes were identified at metaphase I of semi‐fertile F 1 pollen mother cells (PMCs), which indicates homology and chromosome pairing between these two genomes. The C genome had obviously been doubled in two F 2 plants from selfed semi‐fertile F 1 plants. BC 1 plants consisted of 18 C chromosomes and different numbers of one, five and six additional S chromosomes, respectively. Monosomic alien addition lines developed in the present study can be used for B. oleracea breeding and Sinapis alba gene mapping.