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Development of a sequence‐specific PCR marker linked to the Ku gene which removes the vernalization requirement in narrow‐leafed lupin
Author(s) -
Boersma J. G.,
Buirchell B. J.,
Sivasithamparam K.,
Yang H.
Publication year - 2007
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2007.01347.x
Subject(s) - biology , vernalization , genetics , lupinus angustifolius , gene , polymerase chain reaction , genetic marker , population , microsatellite , molecular marker , polymorphism (computer science) , genotype , botany , allele , demography , sociology
Wild types of Lupinus angustifolius require vernalization to promote flowering. Modern domesticated cultivars carry the early‐flowering gene Ku which removes this requirement. A microsatellite‐anchored fragment length polymorphism marker was identified as co‐segregating with the Ku gene in a recombinant inbred line (RIL) population derived from a domesticated × wild‐type cross. DNA sequencing showed that the marker contained a 7 bp insertion/deletion polymorphism, as well as a single nucleotide polymorphism. A pair of sequence‐specific primers was designed and successfully converted the size polymorphism into a simple polymerase chain reaction based co‐dominant marker. This marker is closely linked to the Ku gene, as it co‐segregates with the Ku phenotyping in a population consisting of 106 RILs.