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New interspecific rice genome constructions obtained by symmetric cell fusion
Author(s) -
Feng Y. Y.,
Takahashi H.,
Akagi H.,
Mori K.
Publication year - 2006
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2006.01237.x
Subject(s) - biology , protoplast , hybrid , somatic fusion , oryza sativa , genome , oryza , somatic cell , cultivar , chromosome , interspecific competition , genetics , botany , gene
Newly released rice varieties are mostly progenies of common commercial cultivars and so most gene sources for rice breeding have closely related genotypes. Protoplast fusion technique is a method that can produce hybrid plants in one‐step within a desired cultivar. To introduce new genetic diversity into rice‐breeding materials, hybrids between Oryza sativa L. (AA) and Oryza punctata Kotschy ex Steud. (BBCC) were generated by symmetric protoplast fusion. The genomic distribution in interspecific somatic hybrids was investigated by multi‐colour genomic in situ hybridization. Differences in chromosome numbers were observed not only between different somatic hybrid plants but also within individual hybrid plants. Chromosomal reduction of the A genome was found in two somatic hybrids. These results suggest that it is possible to reconstruct new interspecific rice genomes. Chromosomal reduction in interspecific somatic hybrids may be used to generate breeding lines with novel chromosomal constitutions.