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Development of cleaved amplified polymorphic sequence (CAPS) markers linked to a green rice leafhopper resistance gene, Grh3(t)
Author(s) -
Saka N.,
Tsuji T.,
Toyama T.,
Yano M.,
Izawa T.,
Sasaki T.
Publication year - 2006
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2006.01188.x
Subject(s) - biology , restriction fragment length polymorphism , genetics , japonica , gene , genetic marker , marker assisted selection , genotype , cleaved amplified polymorphic sequence , gene mapping , oryza sativa , chromosome , botany
The chromosomal location of the resistance gene for green rice leafhopper (GRLH), an injurious insect for rice, has been determined and RFLP markers closely linked to this gene have been identified. The susceptible japonica rice variety ‘Nipponbare’ was crossed with a resistant japonica rice line ‘Aichi42’, in which green rice leaf hopper resistance had been introduced from an indica variety ‘Rantaj‐emas2’, and the 100 F 2 plants obtained were used for linkage analysis. The green rice leafhopper resistance gene, Grh3(t) , was mapped between RFLP markers C288B and C133A on chromosome 6 and co‐segregated with C81. Of the RFLP markers tightly linked to Grh3(t) , C81 was converted to a SCAR marker and C133A to a cleaved amplified polymorphic sequence marker that could distinguish the heterozygous genotype to establish an effective marker‐aided selection system for the GRLH resistance gene.