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SSR and SCAR markers linked to the fertility‐restoring gene for a D 2 ‐type cytoplasmic male‐sterile line in wheat
Author(s) -
Li X. L.,
Liu L. K.,
Hou N.,
Liu G. Q.,
Liu C. G.
Publication year - 2005
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2005.01120.x
Subject(s) - bulked segregant analysis , rapd , biology , genetic marker , genetics , cytoplasmic male sterility , common wheat , molecular marker , gene , microsatellite , gene mapping , chromosome , population , allele , genetic diversity , demography , sociology
‘Yi 4060’ is an elite restorer line of a non‐photoperiod‐sensitive D 2 ‐type cytoplasmic male‐sterile (CMS) line of wheat. Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were employed to map one major fertility‐restoring gene (D 2 Rf 1 ) in ‘Yi 4060′. The sterile and fertile DNA pools were established from individuals in BC 6 , based on bulked segregant analysis. One RAPD marker E09 , linked to D 2 Rf 1 , was converted to a SCAR marker and designated as E09‐SCAR 865 . The genetic distance between E09‐SCAR 865 and D 2 Rf 1 is 9.5 cM. Two SSR markers, Xgwm11 and Xgwm18 , were also linked to a D 2 Rf 1 and co‐segregated with E09‐SCAR 865 . The three molecular markers are useful in marker‐assisted breeding of the elite restorer lines for D 2 ‐type CMS lines in wheat.