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Development of a molecular CAPS marker for the self‐incompatibility locus in Brassica napus and identification of different S alleles
Author(s) -
Mohring S.,
Horstmann V.,
Esch E.
Publication year - 2005
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2005.01081.x
Subject(s) - biology , locus (genetics) , genetics , allele , genetic marker , genomic dna , primer (cosmetics) , polymerase chain reaction , restriction fragment length polymorphism , restriction enzyme , genotype , brassica , brassica oleracea , molecular marker , cleaved amplified polymorphic sequence , population , microbiology and biotechnology , dna , gene , botany , chemistry , demography , organic chemistry , sociology
Using primers annealing to S locus sequences the cleaved amplified polymorphic sequences (CAPS) method was applied to develop a marker and to characterize different alleles at the self‐incompatibility locus in Brassica napus. A segregating F 2 population from a cross of a self‐incompatible (SI) and a self‐compatible parent, as well as seven SI lines representing four different S alleles were used. Several primers specific to the S locus in B. oleracea and B. campestris , chosen from the literature, allow polymerase chain reaction (PCR) amplification of genomic DNA. However, only one primer pair amplified a single specific and reproducible PCR fragment of the expected length in B. napus. Digestion with restriction endonucleases revealed polymorphisms for two CAPS markers absolutely linked to the S locus. Using the codominant marker efMboI it was possible to discriminate all three F 2 genotypes. With this marker and an additional marker using another primer pair it was possible to distinguish between three of the four different S alleles and five of the seven SI lines, respectively.