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Determination of incompatibility genotypes in almond using first and second intron consensus primers: detection of new S alleles and correction of reported S genotypes
Author(s) -
Ortega E.,
Sutherland B. G.,
Dicenta F.,
Boskovic R.,
Tobutt K. R.
Publication year - 2005
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.01058.x
Subject(s) - biology , genotyping , genotype , genetics , allele , intron , microsatellite , variants of pcr , gene
The work aimed to develop a reliable and convenient PCR approach for determining incompatibility S genotypes in almond. Initially, genomic DNAs of 24 accessions of known S genotype were amplified with novel consensus primers flanking the first and second introns of the S‐RNase gene. The PCR products separated on agarose showed length polymorphisms and correlated well with the reference alleles S 1‐S 2 3 and Sf. In addition, to improve discrimination between alleles of similar sizes, the same sets of primers but fluorescently labelled were used, and the products sized on an automated sequencer. These fluorescent primers were particularly informative in the case of the first intron, variation in the length of which has not been used previously for S genotyping in almond. Some reference alleles showed the same patterns with first and second intron primers, and others showed a microsatellite‐like trace. Subsequently, the S genotypes of 26 cultivars not genotyped previously and of four of uncertain genotype were determined. An allele described in Australian work as putative S 10 was shown to be a ‘new’ allele and ascribed to S 24 and evidence of five more ‘new’ S alleles was found, for which the labels S 25 ‐S 29 are proposed. This PCR approach should be useful for genotyping in other Prunus crops.