Premium
Production of fertile transgenic Brassica napus by Agrobacterium‐mediated transformation of protoplasts
Author(s) -
Wang Y. P.,
Sonntag K.,
Rudloff E.,
Han J.
Publication year - 2005
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.01015.x
Subject(s) - biology , transformation (genetics) , protoplast , agrobacterium tumefaciens , agrobacterium , brassica , kanamycin , genetically modified crops , botany , gene , transgene , genetics
Abstract A protocol for Agrobacterium tumefaciens‐mediated transformation of Brassica napus mesophyll protoplasts is described. A strain with a neomycin phosphotransferase ( nptII ) gene and a KCS gene under control of a napin promoter was used at co‐cultivation. Transformed protoplasts were regenerated to fertile and morphologically normal transgenic plants. Transformants were confirmed by PCR of the nptII gene and NAP/KCS expression cassette, and Southern blot analysis. Seeds of the transformants showed a changed fatty acid profile: two transformants had a higher erucic acid level and differed significantly from that of B. napus. Genetic analysis of the progeny revealed that the kanamycin resistance introduced was inherited in a Mendelian fashion.