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Production of an interspecific hybrid between Brassica fruticulosa and B. rapa
Author(s) -
Chandra A.,
Gupta M. L.,
Banga S. S.,
Banga S. K.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.01007.x
Subject(s) - biology , brassica , brassica rapa , aphid , pollen , botany , interspecific competition , interspecific hybridization , meiosis , bivalent (engine) , hybrid , ovule , gene , genetics , chemistry , organic chemistry , metal
An interspecific hybrid between a wild species, Brassica fruticulosa (2n = 16, FF) and a crop Brassica species, B. rapa (2n = 20, AA) was synthesized using sequential ovary‐ovule culture on MS medium supplemented with casein hydrolysate. Morphological, molecular and cytogenetic analysis confirmed the true hybrid nature of the offspring. The F1 plants (2n = 18) were intermediate in morphology, highly pollen‐sterile as well as self‐sterile. A maximum of three bivalents per PMC was recorded, but 14 I + 2 II was the most common meiotic configuration. Normal pollen fertility and regular bivalent (18 II) formation was observed in the amphiploid sectors of the hybrid plants. The F 1 hybrid harboured significantly lower aphid populations than the crop Brassica parent.