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Mapping quantitative trait loci and expressed sequence tags related to brown planthopper resistance in rice
Author(s) -
Ren X.,
Wang X.,
Yuan H.,
Weng Q.,
Zhu L.,
He G.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.01003.x
Subject(s) - biology , brown planthopper , quantitative trait locus , suppression subtractive hybridization , genetics , expressed sequence tag , candidate gene , gene mapping , oryza sativa , gene , population , genetic linkage , genome , cdna library , complementary dna , demography , sociology , chromosome
To map genes responsible for brown planthopper (BPH) resistance in rice, a rice genetic map was constructed based on a recombinant inbred line population from a cross between a BPH‐resistant line ‘B5’ and a susceptible variety ‘Minghui 63’. Four quantitative trait loci (QTLs) for BPH resistance were detected. ESTs differentially regulated by BPH feeding were isolated by suppressive subtractive hybridization (SSH) and assigned to chromosomes based on RFLP mapping and searches of the rice genome database. The distribution of ESTs showed some clustering, and some ESTs are related to known QTLs and known BPH resistance genes. These findings suggest that the mapping of differentially induced ESTs may be a useful strategy for the identification of candidate plant defence genes, which could be beneficial in the development of a BPH‐resistant rice variety.