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Radiomutants of chrysanthemum ( Dendranthema grandiflora Tzvelev) of the Lady group: RAPD analysis of the genetic diversity
Author(s) -
LemaRumiñska J.,
Zalewska M.,
Sadoch Z.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.00996.x
Subject(s) - rapd , biology , dendrogram , cultivar , genetic diversity , primer (cosmetics) , botany , polymerase chain reaction , dna profiling , genetics , dna , gene , population , chemistry , demography , organic chemistry , sociology
Random amplified polymorphic DNA (RAPD) markers were used to study the molecular characterization of 10 new radiomutants of chrysanthemum. The original cultivar ‘Richmond’ differed in genetic distance from its Lady group mutants. The analysis of genetic similarity indices revealed low diversity within the radiomutants. The dendrogram obtained after cluster analysis separated the new cultivars as a group that differed from the original cultivar ‘Richmond’. The Lady group cultivars, derived from one original cultivar by radiomutation, could be distinguished from each other by using RAPD markers of only a single primer or sets of two or three primers. Polymerase chain reaction analysis proved the efficiency of the RAPD method for DNA fingerprinting of the original cultivar ‘Richmond’ and its new radiomutants.