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Transfer of powdery mildew resistance from Brassica carinata to Brassica oleracea through embryo rescue
Author(s) -
Tonguç M.,
Griffiths P. D.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.00987.x
Subject(s) - embryo rescue , biology , powdery mildew , brassica oleracea , backcrossing , brassica carinata , rapd , hybrid , interspecific competition , botany , interspecific hybridization , brassica , horticulture , genetics , gene , genetic diversity , population , demography , sociology
Interspecific hybrid plants and backcross 1 (BC 1 ) progeny were produced through sexual crosses and embryo rescue between Brassica carinata accession PI 360883 and B. oleracea cvs Titleist’and‘Cecile’to transfer resistance to powdery mildew to B. oleracea. Four interspecific hybrids were obtained through application of embryo rescue from crosses with B. carinata as the maternal parent, and their interspecific nature confirmed through plant morphology and random amplified polymorphic DNA (RAPD) analysis. Twenty‐one BC 1 plants were obtained through sexual crosses and embryo rescue although embryo rescue was not necessary to produce first backcross generation plants between interspecific hybrids and B. oleracea. All interspecific hybrids and eight of the BC 1 plants were resistant to powdery mildew.