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SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum ‐derived gene Lr24 in wheat: a revision
Author(s) -
Prabhu K.V.,
Gupta S. K.,
Charpe A.,
Koul S.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.00971.x
Subject(s) - gene , rust (programming language) , biology , genetics , programming language , computer science
A sequence characterized amplified region (SCAR) marker tagged to an Agropyron elongatum ‐derived leaf rust resistance ( Lr ) gene Lr19 was validated on 18 known alien Lr gener in near‐isogenic lines (NILs) in the variety ‘Thatcher’, along with three wheat cultivers carrying Lr24 and two carrying Lr19 . The marker was expressed only in the Lr24 lines confirming that the marker tagged the gene Lr24 . The monomorphic expression of the SCAR marker in 10NIL pairs for Lr19 and Lr24 revealed that each NIL pair possessed the same gene, Lr24 . The donor parents used in the NIL pairs for Lr19 (‘Sunstar*6/C80‐1′) and Lr24 (‘TR380‐14*7/3Ag#14′) amplified the same fragment. Nonsegregation for leaf rust in the F 2 population of the cross between the above donor parents confirmed the presence of the same gene in the two parents. Apparently, a genuine parent stock of ‘Sunstar*6/C80‐1’ was not involved in the development of the NIL pairs for Lr19 due to an improper maintence bredding protocol either at source or destination which went undetected in the absence of signs of virulence for either gene in the region.