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Targeted BSA mapping of Scmv1 and Scmv2 conferring resistance to SCMV using PstI/MseI compared with EcoRI/MseI AFLP markers
Author(s) -
Yuan L.,
Duble C. M.,
Muminovic J.,
Melchinger A. E.,
Lübberstedt T.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.00966.x
Subject(s) - amplified fragment length polymorphism , biology , ecori , genetics , bulked segregant analysis , genetic marker , chromosome , gene , gene mapping , genetic diversity , population , restriction enzyme , demography , sociology
In a previous study, two chromosome regions ( Scmv1 and Scmv2 ), conferring sugarcane mosaic virus (SCMV) resistance in maize, were enriched with EcoRI/MseI AFLP ( Eco ‐AFLP) markers (methylation insensitive) by targeted bulked segregant analysis (tBSA). The objective of the present study was to further saturate these two regions with PstI/MseI AFLP (Pst‐AFLP) markers (methylation sensitive) using the same tBSA approach, and to compare the genomic distribution of both Pst ‐AFLP and Eco‐AFLP markers. Out of 470 PstI/MseI primer combinations screened, four Pst ‐AFLP markers were identified in the Scmv1 region (chromosome 6), and none in the Scmv2 region (chromosome 3). These Pst ‐AFLP markers were more closely linked to the Scmv1a gene than any of the Eco ‐AFLP markers, and could be useful for marker‐assisted selection and even map‐based cloning. In addition, Pst ‐AFLP and Eco ‐AFLP markers were dissimilarly distributed in both target regions. Pst ‐AFLP markers were equally distributed across both regions, while Eco ‐AFLPs were clustered in the Scmv2 region.