Premium
RAPD and SCAR markers for powdery mildew resistance gene er in pea
Author(s) -
Janila P.,
Sharma B.
Publication year - 2004
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.2004.00963.x
Subject(s) - powdery mildew , biology , rapd , genetics , marker assisted selection , allele , gene , primer (cosmetics) , population , cleaved amplified polymorphic sequence , molecular marker , polymorphism (computer science) , genetic linkage , genetic marker , botany , restriction fragment length polymorphism , genotype , genetic diversity , chemistry , demography , organic chemistry , sociology
In pea, a single recessive gene ( er ) on linkage group 6 confers resistance to powdery mildew caused by Erysiphe pisi. The present study aims to identify molecular markers linked to the er gene. Screening of the powdery mildew‐resistant cultivar ‘DMR11’ and its susceptible nearisogenic line for polymorphism revealed linkage of two RAPD primers (OPO‐02 and OPU‐17) to the er gene and a sequence characterized polymorphic region (SCAR) primer, ScOPD‐10 650 with er in a population of 83 F 2 plants in the order: OPU‐17 ‐ er ‐ ScOPD‐10 650 ‐ OPO‐02. The markers ScOPD‐10 650 and OPU‐17 being coupled with the allele causing resistance would substantially increase the efficiency of marker‐assisted selection in peabreeding for powdery mildew.