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Multiple changes in tobacco mitochondrial DNA populations during tissue culture
Author(s) -
KANAZAWA A.
Publication year - 1998
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1998.tb01975.x
Subject(s) - mitochondrial dna , biology , subgenomic mrna , dna , tissue culture , mitochondrion , southern blot , microbiology and biotechnology , genetics , genome , gene , in vitro
Changes in the composition of mitochondrial DNA (mtDNA) in tobacco during the processes of long‐term tissue culture and regeneration were detected by DNA gel blot analyses using several mtDNA fragments as probes. The changes in mtDNA were identified by differential accumulations of mtDNA fragments in cultured cells that were hybridized with probes atp6, cob , and nad3‐rpsl2. The changes detected by each probe did not appear to be linked, since they were different with each probe and in different subcultures of calli. Based on the current results and previous detailed analyses of the changes detected by atp6, a possible model that explains the alterations in mtDNA during tissue culture is presented. In this model, the changes are attributed to multiple shifts in the equilibrium that was maintained between different subgenomic mtDNA molecules.