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Variation in seed proteins from mutagen‐treated cultivars and selected lines of Vigna unguiculata L. Walp
Author(s) -
Osanyinpeju A. O.,
Odeigah P. G. C.
Publication year - 1998
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1998.tb01954.x
Subject(s) - vigna , biology , cultivar , germplasm , storage protein , gel electrophoresis , polyacrylamide gel electrophoresis , sodium azide , globulin , molecular mass , albumin , sodium , horticulture , botany , microbiology and biotechnology , gene , biochemistry , chemistry , organic chemistry , immunology , enzyme
The storage protein profiles of the seeds of two IITA cowpea cultivars (‘IT84E–124’ and ‘Vita 7’) exposed to three mutagens—sodium azide (NaN 3 ), ethyl methane sulphonate (EMS) and 60 Co gamma rays—and those of 18 selected M 3 lines were investigated by sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis (PAGE). The total seed protein, globulin and albumin fractions showed differences in number and intensity of subunit bands, but the least differences were found in the albumin fraction. Both high and low molecular weight bands were observed, the highest being 94.5 kDa and the lowest 12.0 kDa.‘Vita T showed less variability compared with ‘IT84E–124’, as indicated by the relative similarity indices (S.I.) of the two cultivars. The lowest S.I. of 0.200 was found among ‘IT84E–124’ lines while the lowest S.I. among ‘Vita T lines was 0.4706. A number of lines with particular traits were found to be characterized by the presence of specific polypeptide bands. This study demonstrates that induced mutation could create additional variability to supplement existing germplasm and that SDS‐PAGE is a useful tool for discriminating and estimating genetic similarities among selections.