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Identification of two new genes conferring resistance to rice blast in the Chinese native cultivar ‘Maowangu’
Author(s) -
Pan Q. H.,
Wang L.,
Ikehashi H.,
Yamagata H.,
Tanisaka T.
Publication year - 1998
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1998.tb01443.x
Subject(s) - biology , gene , cultivar , genetics , chromosome , recombination , genetic linkage , allele , magnaporthe grisea , botany , oryza sativa
The Chinese native rice cultivar ‘Maowangu’ expresses a high level of resistance to many races of rice blast ( Pyricularia grisea ) collected from North China and Japan. ‘Maowangu’ was crossed with 10 Japanese differential cultivars and the susceptible Chinese cultivar ‘Lijiangxintuanheigu’ (LTH). Allelism tests were conducted in the F 2 populations with rice blast races. The resistance of ‘Maowangu’ was governed by two dominant genes which were non‐allelic to the resistance genes at seven loci: Pi‐a, Pi‐i, Pi‐k, Pi‐z, Pi‐ta, Pi‐b , and Pi‐t. To identify the two resistance genes, two F 3 lines of ‘Shin 2/Maowangu’ segregating 3R:1S were selected for linkage tests in 1994. One was linked to marker genes C and Amp ‐3 on chromosome 6 with recombination frequencies of 35.8 ± 6.4% and 42.1 ± 6.2%, respectively, and the other to Amp ‐1 on chromosome 2 with a recombination frequency of 37.6 ± 6.0%. To confirm these results, two F 3 lines of ‘LTH/Maowangu’ were selected for linkage tests in 1995. The one was linked to Amp ‐3, and other was linked to Amp ‐1, with recombination frequencies of 36.9 ± 3.1% and 34.3 ± 3.2%, respectively. The two genes on chromosomes 6 and 2 were designated Pi 13(t) and Pi 14(t), respectively.