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Genetic control of alcohol dehydrogenase, malate dehydrogenase, and phosphoglucomutase isozymes in lima bean ( Phaseolus lunatus L.)
Author(s) -
BI I. Zoro,
Maquet A.,
Wathelet B.,
Baudoin J. P.
Publication year - 1997
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1997.tb02175.x
Subject(s) - malate dehydrogenase , phosphoglucomutase , biology , isozyme , alcohol dehydrogenase , phaseolus , biochemistry , genetics , locus (genetics) , enzyme , gene , botany
A suitable electrophoretic separation method for alcohol dehydrogenase (ADH). malate dehydrogenase (MDH). and phosphoglucomutase (PGM) from P. lunatus has been developed. Two loci ( Adh‐2 and Pgm‐2 ) showed codominant inheritance and fitted Mendelian ratio. ADH isozyme banding patterns indicate a dimeric quaternary structure. while those of PGM were in agreement with a monomeric nature. The cytoplasmic location of two MDH isozymes ( Mdh ‐1 and Mdh ‐2) selectively inactivated by homogenization in an ascorbic acid solution was demonstrated. However. distorted ratios were observed for Mdh‐ 2 segregation. On the basis of MDH isozyme banding patterns observed in five progeny families, il is suggested that this enzyme system is a dimeric protein encoded by at least three codominant genes ( Mdh ‐1 . Mdh ‐2 and Mdh‐3 ). Joint segregation tests between pairs of segregating loci ( Adh‐ 2 , Mdh‐2 , and Pgm‐ 2) indicated that each of them is inherited independently.