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Interspecific crosses between Pelargonium × hortorum and P. quinquelobatum using embryo rescue and molecular characterization of hybrids by an endogenous chs probe
Author(s) -
DenisPeixoto L.,
Cadic A.,
Renou J. P.
Publication year - 1997
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1997.tb02174.x
Subject(s) - biology , embryo rescue , pelargonium , hybrid , petal , botany , restriction fragment length polymorphism , subgenus , polymerase chain reaction , gene , microbiology and biotechnology , genetics , interspecific hybridization , genus
Interspecific hybridizations were performed between pelargonium × hortorum (subgenus Ciconium ) and a yellow‐flowered species. Pelargonium qitinquelobatum(subgenus Eumurpha ). Hybrids, using embryo rescue, were obtained only when P. quinquelobatum was used as the female parent. A polymerase chain reaction (PCR) cloned Pelargonium chs gene fragment was used as probe to characterise hybrids hy restriction fragment length polymorphism (RFLP) analysis. Chromatographic flavonoid analysis showed that hybrids could synthesize both flavonol glycosides and anthocyanin in the petals which were pale yellow to pale pink from the proximal to the distal part.