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Molecular characterization of fertile and sterile cytoplasms in Beta spp.
Author(s) -
Sadoch Z.,
Goc A.
Publication year - 1997
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1997.tb01023.x
Subject(s) - cytoplasmic male sterility , biology , mitochondrial dna , restriction fragment length polymorphism , sugar beet , restriction enzyme , genetics , software maintainer , sterility , genome , gene , polymerase chain reaction , horticulture
Mitochondrial DNA (mtDNA) from sugar beet carrying fertile (F) and male sterile (CMS) cytoplasms, and from male sterile accession of Beta maritima collected in Brittany (France) were characterized and compared by restriction fragment length polymorphism (RFLP) and Southern hybridization with cox II. The F and CMS cytoplasms could be clearly distinguished from each other by RFLP when Xho I, Eco RI and Bam HI endonucleases were used. Southern hybridization with the cox II gene provided further evidence that mitochondrial genome organization differs between fertile and sterile plants. All cytoplasmic male sterile lines from different breeding stations showed the same restriction and hybridization patterns, which confirms the uniformity of mitochondrial genomes within the materials used for hybrid seed production in several European countries. No visible differences were found between the maintainer lines studied. However, comparisons of Xho I restriction profiles of mtDNA from maintainer lines and from fertile monogerm populations revealed slight differences, which were reflected by the appearance of a unique 0.9 kb fragment in the latter. Analysis of mtDNA from male sterile plants of the wild beet B. maritima showed different restriction and hybridization patterns in comparison with normal and sterile sugar beet cytoplasms. This shows the unique nature of cytoplasmic male sterility in this species.