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High‐lysine mutants of rice, Oryza sativa L.
Author(s) -
Kumamaru T.,
Sato H.,
Satoh H.
Publication year - 1997
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1997.tb00990.x
Subject(s) - endosperm , lysine , mutant , oryza sativa , biology , amino acid , biochemistry , essential amino acid , threonine , histidine , food science , gene , serine , enzyme
Abstract In order to improve the nutritive value of rice protein, it is necessary to increase the level of lysine and threonine. Ten high‐lysine mutants were selected from N‐methyl‐N‐nitrosourea‐induced (MNU‐induced) mutant lines, using high‐performance liquid chromatography for amino acid analysis. The lysine content of these mutants ranged from 5.10% to 6.38% of total amino acids, while that of the original varieties was about 4%. All high‐lysine mutants also had a greatly increased histidine content and a floury endosperm. An increase in lysine content of the albumin/globulin fraction was characteristic of the MNU‐induced high‐lysine mutants. The segregation ratio of normal to floury endosperm in the F2 seeds from a cross between ‘Kinmaze’ and a high‐lysine mutant fitted the expected 3:1 ratio. The high level of lysine and the floury endosperm may be controlled by the same gene.