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Evaluation of random amplified polymorphic DNA (RAPD) markers in Hevea brasiliensis
Author(s) -
Varghese Y. A.,
Knaak C.,
Sethuraj M. R.,
Ecke W.
Publication year - 1997
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/j.1439-0523.1997.tb00973.x
Subject(s) - rapd , hevea brasiliensis , upgma , biology , hevea , genetic marker , genetics , genetic distance , genetic diversity , genotype , genetic variation , gene , population , natural rubber , chemistry , demography , organic chemistry , sociology
The applicability of random amplified polymorphic DNA (RAPD) markers in the cultivated rubber tree, Hevea , was evaluated using 43 decamer oligonucleotide primers in a set of 24 clones selected in different South‐East Asian countries. A total of 220 0.35–3.5 kb DNA fragments were amplified, of which 111 were polymorphic. Of these, 80 fragments (RAPD markers) which were repeatable and clearly scorable across all genotypes were used to estimate genetic distances among the clones tested. The estimated genetic distances ranged from 0.05 (RRII 308 and PB 5/51) to 0.75 (RRIC 100 and SCATC 88–13). A mean genetic distance of 0.5 indicates a rather high genetic variability among the tested clones. As expected, because of the breeding history of Hevea , UPGMA cluster analysis and Principal Coordinate Analysis (PCoA) indicated the absence of a distinct geographical grouping. The possible application of RAPD markers for clone identification and also for analysis of genetic relationships among Hevea clones is discussed.